RNA

Part:BBa_K3096052:Design

Designed by: Jakob Keck, Patrick Mueller, Lina Widerspick, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)


CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Trouble with synthesis of repeats. Target sequences had to lie in direct neighbourhood to a PAM sequence.


Source

Synthesis

References